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Classical methods of protein purification can be divided into methods that utilize chemical properties of the protein including solubility [2], physical characteristics, e.g
Mannitol, with a cross-sectional hydrated diameter of 6.5 is a small solute that is used as a marker of total intestinal surface area available for absorption, whereas lactulose (with a larger cross-sectional diameter of 9.5 ) is used to assess the paracellular permeability through the tight junctions normalized to the total available intestinal surface [32], with results expressed as a ratio of the permeability of mannitol to lactulose
This compounds across multiple exposures, which is why strict cold chain discipline matters from the moment of reconstitution
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